The results revealed that the antifungal compound succeed isolated in its Substance B form from methanol extract from the interior of the root. Substance B showed the highest level of antifungal activity through inhibiting germination and inhibiting of germination tube growth of Fusarium sp. This was shown by the highest percentage inhibiting of germination (66,67%), and the highest percentage inhibiting of germination tube (66,03%). The inhibition zone of hyphal growth of Ganoderma lucidum macroscopically from the antifungal compound was observed at a concentration of 1800 μg/ml. Microscopically, in the area of contact with the antifungal compound, hyphal curling and distorting of tips took place at a concentration of 1500 μg/ml one day after application of the antifungal compound. Based on the analysis of GC-MS spectra, the antifungal was identified as p-Methoxybenzylidene p aminophenol in the category of phenolic compounds.
Fahrunnida, & Pratiwi, R. (2015). Kandungan saponin buah, daun dan tangkai daun belimbing wuluh (Averrhoa bilimbi L.) [The content of saponin in fruits, leaves and petioles of belimbing wuluh (Averrhoa bilimbi L.)]. In Sajidan, C. Muryani, M. G. Rindarjono, P. Karyanto, M. Masykuri, Maridi, .......... Suciati (Eds.), Towards conservation and sustainable use of natural resources: A perspective of education, biology, geography and environmental science. Paper presented at Proceedings of the National Seminar of Conservation and Utilization of Natural Resources, Surakarta, 13 January (pp. 220-224). Surakarta, ID: Faculty of Teacher Training and Education, Universitas Sebelas Maret. Retrieved from http:////kpsda/article/view/5378 website
This research was conducted with the maceration extraction continuing by test toxicity against the shrimp larvae of A . salina Leach (BSLT). The result of the toxicity test then proceeds with the test reagents and phytochemicals with thin layer chromatography for positive test of compound phytochemicals. Thin-layer chromatography using eluen n-hexane: ethyl acetate (7: 3) to ethanol extract and n-hexane: ethyl acetate (17: 3) for n-hexane extract. Data on toxicity of A. salina Leach analyzed by probit analysis to find out the value of LC 50 on each extract.